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This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab. Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.

Plant genetics. --- Plant Genetics and Genomics. --- Plants --- Genetics --- Genòmica --- Genètica vegetal --- Fitogenètica --- Genètica de les plantes --- Genètica --- Genètica molecular vegetal --- Nombre de cromosomes de les plantes --- Millorament selectiu de plantes --- Genètica molecular --- Metagenòmica --- Nutrigenòmica --- Genomes

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Sesame. --- Beniseed --- Orientale sesame --- Sesamum indicum --- Sesamum orientale --- Oilseed plants --- Sesamum --- Genètica vegetal --- Fitogenètica --- Genètica de les plantes --- Genètica --- Genètica molecular vegetal --- Nombre de cromosomes de les plantes --- Millorament selectiu de plantes

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This book describes the current state of international grape genomics, with a focus on the latest findings, tools and strategies employed in genome sequencing and analysis, and genetic mapping of important agronomic traits. It also discusses how these are having a direct impact on outcomes for grape breeders and the international grape research community. While V. vinifera is a model species, it is not always appreciated that its cultivation usually requires the use of other Vitis species as rootstocks. The book discusses genetic diversity within the Vitis genus, the available genetic resources for breeding, and the available genomic resources for other Vitis species. Grapes (Vitis vinifera spp. vinifera) have been a source of food and wine since their domestication from their wild progenitor (Vitis vinifera ssp. sylvestris) around 8,000 years ago, and they are now the world’s most valuable horticultural crop. In addition to being economically important, V. vinifera is also a model organism for the study of perennial fruit crops for two reasons: Firstly, its ability to be transformed and micropropagated via somatic embryogenesis, and secondly its relatively small genome size of 500 Mb. The economic importance of grapes made V. vinifera an obvious early candidate for genomic sequencing, and accordingly, two draft genomes were reported in 2007. Remarkably, these were the first genomes of any fruiting crop to be sequenced and only the fourth for flowering plants. Although riddled with gaps and potentially omitting large regions of repetitive sequences, the two genomes have provided valuable insights into grape genomes. Cited in over 2,000 articles, the genome has served as a reference in more than 3,000 genome-wide transcriptional analyses. Further, recent advances in DNA sequencing and bioinformatics are enabling the assembly of reference-grade genome references for more grape genotypes revealing the exceptional extent of structural variation in the species.

Plant genetics. --- Plant breeding. --- Agriculture. --- Plant Genetics and Genomics. --- Plant Breeding/Biotechnology. --- Farming --- Husbandry --- Industrial arts --- Life sciences --- Food supply --- Land use, Rural --- Crops --- Agriculture --- Breeding --- Plants --- Genetics --- Grapes --- Genetics. --- Grape --- Grape vines --- Grapevines --- Vitis --- Wine grapes --- Vitaceae --- Enocyanin --- Viticulture --- Genètica vegetal --- Fitogenètica --- Genètica de les plantes --- Genètica --- Genètica molecular vegetal --- Nombre de cromosomes de les plantes --- Millorament selectiu de plantes

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Plant Chemical Genomics: Methods and Protocols , expert researchers in the field detail many of the techniques used for identification of bioactive compounds from a large selection of fields in plant biology including plant pathogenesis, immune responses, small RNA processing, endomembrane trafficking, lipids, plant hormone signaling and cell wall. The presentation of these and other examples as well as synthetic chemistry, computation and target identification provides a comprehensive overview of the practical aspects of chemical biology that are possible in plant systems. Small molecules and natural products that produce a phenotype in plants have been known for decades. The agrichemical industry has utilized small molecules based on known natural molecules such as auxins, cytokinins, abscissic acid and other growth regulators. In recent years, the screening and characterization of novel small molecules has enhanced our understanding of protein function, metabolic, signaling and endomembrane pathways, and their interactions in basic plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Botany. --- Plant genetics. --- Plant Sciences. --- Plant Genetics and Genomics. --- Plants --- Genetics --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Floristic botany --- Genomes --- Genètica vegetal --- Manuals de laboratori --- Protocols clínics $6$2 thub --- Tècniques de laboratori --- Fitogenètica --- Genètica de les plantes --- Genètica --- Genètica molecular vegetal --- Nombre de cromosomes de les plantes --- Millorament selectiu de plantes --- Genoma --- Genoma humà --- Genòmica

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Botany. --- Genetics. --- Biotechnology. --- Chemical engineering --- Genetic engineering --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Botanical science --- Floristic botany --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Natural history --- Plants --- Genètica vegetal --- Fitogenètica --- Genètica de les plantes --- Genètica --- Genètica molecular vegetal --- Nombre de cromosomes de les plantes --- Millorament selectiu de plantes --- Tec (Botànica)